English
Subcultura refertur ad processum dividendi culturam in partes parvas, eas in alterum vasculum culturae rursus inoculandum (uter), et postea culturam. @___@ solent consumables in colendis cellulis. Quomodo ergo cellulas in uasculo subculturas? Plerumque, cellulae in culturae cella ampullae in densum monolayrum crescunt et radicaliter saturantur. Ut cellas efficiat ut cellularum numerum effingere et ampliare pergant, operationes transitus explere necesse est. Cellulae suspensae directe separari possunt, cellulae autem adhaerentes prius concoquendae sunt quam separari possunt. Fere enzymes proteolyticae (ut trypsin) adhaerentes cellulas in cellulas singulas digerunt. Acidum ethylenediaminetetraaceticum (EDTA) addi etiam potest ad concoctionem meliorem. Cellulas observa donec cellulae rotundae fiant et a muro utrem seiungas. Hic processus saepe 5-15 minuta sumit. Ut filicum cellularum vitando, ne ampullas culturae cellularum attingas in processu digerendi cellulas.Cellulae culturae ampullas
Pro cellulis concoquendis maxime difficilis, solutionem digestionis trypsin addere potes et cellas ad 37°C pones. faciliorem concoctionem. Postquam cellulae a pariete utre separati sunt, statim medium integram continentem seri ad concoctionem sistendam adde. Quidam servo in sero pancreate inhibere possunt. Actio enzyme. Fere centrifugatio non est necessaria. Si cellulae serum liberum vel humilem-serum medium requirunt, centrifugium ad 125000g per 5 minutas, medium discontinuum abiicias, novum medium aptum adde et cellulas ante subculturam sensim commiscebis.
Ratio transiens cellula fere 1:2 - 1:20, genus cellulae fretus. In processu obeundi, trypsin cellam membranam cellularum quarumdam laedet. Cellam rasoriis uti potes ad cellulas leniter radere, medium debitum addere, sensim pipettam ad cellulas miscere, easque ad novam culturam lagonam transferre.
The cell passaging ratio is generally 1:2-1:20, depending on the cell type. In the process of passaging, trypsin will damage the cell membrane of some cells. You can use a cell scraper to gently scrape the cells, add an appropriate amount of medium, gently pipette to mix the cells, and transfer them to a new culture flask.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.