English
A ___@ iacuit unus vel multi-strati structurae culturae cellae consumables. Per processum culturae cellae, opus est incrementum status cellularum tempore capere ante ad gradum proximum progredi. Numerus cellularum viabilium ab trypan caeruleo tinctu deprehendi potest. Methodus specificae operationis haec est: 1. Cellulae adhaerentes medium aspirant, solutionem concoctionis addunt, et in incubatore opportuno tempore concoquunt. Postquam cellulae rotundae factae sunt, concoctio terminata est addendo volumen medium aequali vel maius quam digestum. Post centrifugam, cellula media culturae suspensio facta est; cellam officinam
2. Solutio trypanum caeruleum accipe 0.5ml, 0.3ml PBS et 0.2ml cellam suspensionis ac bene misce. Tum mixtura 1-2 min relicta est. Utere pipettis capillaribus vel 10ul parvis pipettis apice ut parvam quantitatem mixtionis hauriendam et in hemocytometrum injicias;
3. Numera saltem 1000 cellulas et cellas maculatas numero computa;
4. Lava lamina numeralis et opercula cum aqua ultrapura et 75% ethanol;
5. Cellulae normales non maculabuntur, nisi postquam cellulae moriuntur, membrana cellae permeabilitas fit maior, cellas trypan caeruleae intrat, et cellulae caeruleae apparent. Mixtio non debet pro nimium diuturna, secus normales cellulae vivae etiam fucum suscipient, quae numerationem accurationem afficiet;
6. Cellula viability (%) = (numerus cellularum - numerus cellularum maculatarum)/numerus cellularum* 100;
7. Sicut cellae modum computandi, trypan caeruleum maculat magnum errorem habet. Solum apoptosim primos gradus deprehendere potest et differentiam inter catervam moderatricem et coetus experimentalem ostendere non potest.
In processu colendi cellas in officina cella, sunt semper aliquae cellae quae ob varias causas moriuntur. Domito numero cellularum viventium et viabilitatis cellae clavis est operatio experimentorum culturae cellae et modus efficax ad progressum experimenti efficere.
In the process of culturing cells in a cell factory, there are always some cells that die due to various reasons. Mastering the number of living cells and cell viability is a key operation in cell culture experiments and an effective way to ensure the progress of the experiment.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.