English
Contaminatio mycoplasma valde usitatissimum est problema culturae cellularum in officinarum cellae. Dissimiles aliae contaminationes, cellae mycoplasma-contaminatae plerumque non nubilum fiunt, ita difficile est nudo oculo iudicare. Quattuor modi sunt determinare num cellae mycoplasma contaminatae sunt:
1. DNA fluorescens maculavit methodum inficiens
The DNA fluorescens methodus innititur principio quod fucus fluorescens Hoechst33258 potest ligare ad regionem AT basi divitem in DNA mycoplasmatis. punctis fluorescentibus sunt mycoplasma DNA in regionibus basi AT divites.
2. PCR technologia
Primarii specifici ordinantur secundum sequentia conservata in genome Mycoplasma, acidum nuclei specimen probandi ampliatur, et diagnosis fit per ampliationem operis magnitudinem analysis. PCR detectio technologia adhibetur ad detectionem contagionis mycoplasmatis, cum brevibus cyclis, magna sensibilitate, bona specie, operatione simplici, ac permulta exempla exemplorum uno tempore deprehendere potest.
3. Enzyme connexum immunosorbent tentamentum (ELISA)
ELISA in detectione contagionis mycoplasmatis, cum bona specie et sensibilitate, et detectionem permultorum exemplorum uno tempore perficere potest. Characteres simplices, rapidae quantitatisque detectionis qualitates habet.
4. Generaliter, cellae in officinarum cellularum per 48 ad 72 horas excultae sunt. Priusquam cellae paene confluentes sunt, cellae cum trypsine digerunt ut cellulam suspensionem, fixam, immersam et ante observationem divisam faciant.
Hae quattuor modi deprehenduntur communiter cum mycoplasma contaminationis in cellulis officinarum deprehenduntur. Nunc plura quam 20 genera mycoplasmatis notae sunt cellulis contaminandis, quarum plus quam 95% mycoplasma orale sunt, ideo operarii operam dare debent ad operationem asepticam cum cellulas culturae.
The above are four commonly used detection methods when detecting mycoplasma contamination in cell factories. At present, there are more than 20 kinds of mycoplasma known to contaminate cells, of which more than 95% are oral mycoplasma, so operators should pay attention to aseptic operation when culturing cells.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.