English
Cellulae culturae ampullascellulae culturae species sunt consumptoriae quae late in foro utuntur et maxime aptae sunt ad culturam cellularum adhaerentium. Apud eos, haec consumabilis etiam in transitu fibroblastarum murium adhibetur.
Mus fibroblasts speciales sunt cellulae adhibitae ad non-medicas usus sicut ad investigationes scientificas vel applicationes industriales. Fibroblastae munera strenua habent et cytoplasmum basophilicum debilem. Interdum synthesim et secretionem operationum manifestos habent. Sub certis conditionibus, mutuam transformationem fibrarum cellulis consequi possunt. Recepta fibroblasta, stabiliri debent in incubatore per 4 circiter horas et tunc exculta vel passa secundum densitatem cellulae, mutato medio. Operatio transitus hos gradus sequitur:
1. Medium aspira in culturae cellularum originalium lagonam, cellulas eluo bis cum PBS quiddam, et 1~2 ml 0.25% EDTA ad concoctionem trypsin adde (attendite ad tempus digestionis, plerumque intra 1~2min moderatum).
2. Concoctionem sub microscopio observa. Cum cellulae marginem recusat et parieti adhaeret (nec commendatur digestio donec cellae natant), trypsin removere, medium perfecti 6~8ml adde, stratum cellularum sensim sufflaturum, et stratum cellularum sufflare tentare et explodere. .
3. Tolle suspensionis cellae partem, et transfer ad novam culturam cellularum lagonem, medium congruum adde completum, et incubatorem cole.
4. Attende mutationem valoris medii pH, regulariter medium muta, et subculturam vel frigidum repete cum densitas cellae attingit 70-80%.
Praecipuum munus Cellae culturae ampullas in cellam transitum est. cellas reponunt et praebent cellularum ambitum cohaerentem incrementum. Animadvertendum est, si paries cellae fractae vel receptae cellas diffluerent, significat cellulas contaminatas esse, et elit tempore contingi debere.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.