English
Lysis cellula est methodus experimentalis momenti mutandi cellam permeabilitatis et actionis. Significat quod post cellam virus infectam, virus nutrientibus in cellula hospitis utitur ad suam prolem summam componendam, et deinde virus fetus cellam hostiam destruit et ex cellulis emittitur. In Lysis cellis experimenta, ___@ communia sunt consumbilia. Gradus cella lysis haec sunt: 1. Medium culturae effunde, et fibula culturae cellae utrem bibulas chartas concitabit, ita ut charta bibula medium culturam absorbet (vel utrem aliquantisper erectum ponet, ut medium culturae residua fluere ad fundum lagenae permittat. tum utere tibia ut eam sugeret ).cellulae culturae ampullae
2. Adde 3ml ex 4℃ prae refrigeratum PBS (0.01M pH7.2
～7.3) unicuique lagenam cellularum. Cellulas planas pone et leniter 1 min ut cellulas lavandas excutias, et solutionem lavationis abiicias. Reitera operationem praedictam bis, cellulas lavans ter ad abluendum culturae fluidum. PBS abiicias et culturam lenticulam in glacie pone.3. Adde 10ul PMSF (100mM) ad quiddam 1ml Lysis, bene excute et in glacie locum. (PMSF excutienda est donec nulla crystalla prius misceantur cum solutione lysis.)
4. Adde 400ul lysis quiddam PMSF continens unicuique ampullas cellularum, et lyse super glaciem per 30 minutas. Ut cellulas plene adstringat, crebro utrem oportet concuti.
5. Post lysis utere mundo rasorio ad cellulas in una parte culturae lenticulam (actio debet esse velox), utere sclopetis ad strages cellularum movendas et ad fistulam centrifugii 1.5ml lysatam. (Tota operatio glaciei quam maxime facienda est.)
6. Centrifugium 12000 rpm ad 4°C pro 5 min. (Converte machinam in antecessum ad frigus)
7. Centrifugas supernatantes in tubum centrifugium transfer pro 0,5 min et repone sub -20°C.
non solum experimenta cellae lysis, sed etiam ad cellam mediae et texti culturae in officina laboratorium, sicut Vero cellae. , HEK 293 cellae, cellae CAR-T, CHO cellae, cellulae insecti, etc.
Cell culture flasks Cellae culturae ampullae
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.