English
Haec ___@ est consumabilis quae saepe adhibetur ad culturam cellularum adhaerentium. Per experimentum, necesse est ut cellae statum incrementum capiant in tempore reali et congruentes adaptationes iuxta condicionem specificam constituant. Cellula viability MTT methodo deprehendi potest ad incrementum cellularum determinare. MTT methodus, quae etiam MTT colorimetrica methodus nota est, methodus est ad cellulam superessendam et incrementum detegendam. Principium detectionis est quod dehydrogenas succinatas in mitochondria cellarum vivarum exogenosas MTT reducere potest ad formazanda crystallina caeruleo-purpurea solvenda et in cellis deposita, cum cellulae mortuae nullum tale munus habent. Dimethyl sulfoxida (DMSO) formazan in cellis dissolvere potest, eiusque valor lucis effusio metiri potest ab enzyme-coniuncto immunosorbenti primordium in 570nm necem, quae indirecte numerum cellularum viventium reflectere potest. Intra certum numerum cellularum, quantitas formationis crystalli MTT numero cellularum proportionalis est. Haec methodus late adhibita est in deprehensione factorum actuum quorumdam biologice activorum, magnarum anti-tumoris medicamentorum protegentium, cytotoxicitatis experimentorum et tumoris radiosentivitatis primordium. Indolem sentiendi et oeconomiae notatur.cell officina
Priusquam experiatur viability cellularum in cella officina, gravissima gradatim formula MTT est. Typice, intentio MTT in hac methodo 5 mg/ml est. Ideo, 0.5 g MTT ponderari potest, dissolvi in 100 ml phosphatis salinae optinet (PBS) vel phenolum medium liberum rubrum, percolatum cum 0.22 µm colum ad bacteria in solutione removendum et ad 4°C in tenebris repositum . . In praeparatione et repositione, vas aluminium foil involvere stude.
Cum MTT methodo utens ut metiaris actionem cellam in officina cella, notandum est MTT carcinogenicum esse, ita diligenter utens. Haec methodus solum adhiberi potest ad numerum cellularum relativum et viability relativum deprehendere, non autem numerus cellularum absolutarum. Cum probans eventus cum lectore microplato, ut linearitas eventus experimentalis caveat, MTT absorbance in latitudine 0-0.7 quam maxime debet esse.
When using the MTT method to measure the cell activity in the cell factory, it should be noted that MTT is carcinogenic, so be careful when using it. This method can only be used to detect relative cell number and relative viability, but not absolute cell number. When testing the results with a microplate reader, in order to ensure the linearity of the experimental results, the MTT absorbance should be in the range of 0-0.7 as much as possible.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.