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2. Efni og aðferðir
2.1. Viðhald á Huh7.5 frumum
Huh7.5 frumuræktum var viðhaldið í frumuræktunar T-flöskur í miðli sem inniheldur sermi (SCM): Dulbecco's Modified Eagle Medium (DMEM) (Gibco, Paisley, Bretlandi) sem innihélt 4 mM GlutaMAX og bætt við með 10% nautgripasermi frá fóstur (Sigma, St. Louis, MO, Bandaríkjunum), 100 U/mL penicillín og 100 µg/mL streptomycin (Sigma). Í T175 flöskum (Nunc, Hróarskeldu, Danmörku) og þrílaga ræktunarflöskum (Nunc), voru Huh7.5 frumur yfirfarnar á 2–3 daga fresti. Öllum frumuræktunum var haldið við 37 °C og 5% CO2.
2.2. HCV Virus Stocks
Tveir raðstaðfestir stofnar af arfgerð 5a raðbrigða SA13/JFH1core-NS5B [28], ræktaðir í einlaga Huh7.5 frumuræktum í T-flöskum, voru notaðir í þessari rannsókn. Þriðja leiðarveirustofninn með sýkingartítli upp á 5,8 log10 FFU/ml, ræktaður við aðstæður sem innihalda sermi (SC) [39], var notaður til að sáningu á CelCradle™ ræktun, hrista flöskuræktun og fyrir víruseinkennistilraunir. Fjórða vírusstofn með sýkingartítli upp á 5,1 log10 FFU/mL [32], ræktaður við SF aðstæður [39], var notaður fyrir víruseinkennistilraunir.
Heimild frá: https://www.mdpi .com/2076-393X/10/2/249/htm
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.