English
Is fadhb choitianta é clumping cille nuair a úsáidtear fleascáin chultúir cille do chultúr na gceall. Cuirfidh bearradh na gceall isteach ar ghnáthfhás agus atáirgeadh cealla. Ansin, conas an fhadhb seo a réiteach?
Ar an gcéad dul síos, ní gá go gcuirfeadh bearradh cille isteach ar phróiseas cultúir na gceall. Má tá imlíne soiléir ag an gcill agus comhlacht cille tréscaoilteach, cnuasaithe go haonfhoirmeach, cruthaíonn sé go bhfuil an chill ag fás go maith. Mar sin féin, i bhformhór na gcásanna, tá imlíne doiléir ag na cealla cnuasaithe, tarchur solais lag, agus fiú syncytia, in éineacht le smionagar cille, rud a chruthaíonn go bhfuil na cealla senescent nó galair agus go bhfuil siad i ndroch-chaoi. Tá go leor bealaí ann chun fadhb na clumping cille a réiteach:
1. Mura ndéanann an clumping cille difear don fhás, níl aon fhadhb ann, ach tá sé níos trioblóideacha a chomhaireamh. Le linn an díleá, sula n-athraíonn na cealla ó chruth polagánach go cruth cruinn, sconna ar bhalla taobh an fhleascáin chultúir cille le do lámh chun na cealla a bhaint as an mballa (is fearr gan séideadh, nach bhfuil go maith don. cruth agus fás na gceall). Chuig
2. Má bhraitheann tú DNA na gceall marbh, cuir cúpla braon de DNA steiriúla (1mg/ml tuaslagtha in uisce) leis an bhfionraí cille chun an snáithe DNA a bhriseadh. Séid na cealla go réidh chun damáiste fisiciúil don chillscannán a chosc.
3. Má tá an tsúil naked le feiceáil ar na clumps cille, lig do na cealla seasamh ar feadh thart ar leath nóiméad, agus ansin pípéad leath uachtarach na fionraí cille gan clumps le haghaidh sliocht. Mura bhfuil an tsúil naked le feiceáil ar na clumps cille, níl aon mhodh deighilte go háirithe maith ann faoi láthair, agus ní féidir na cealla seo a dhíchur go mall ach amháin tar éis feabhas a chur ar riocht na gcealla. is féidir é a chóireáil de réir na modhanna thuas. Le linn an phróisis chultúir cille, is gá stádas fáis na gcealla a urramú in am agus coigeartuithe comhfhreagracha a dhéanamh de réir an staid.
If the cells in the cell culture flasks appear clumping, they can be treated according to the above methods. During the cell culture process, it is necessary to observe the growth status of the cells in time and make corresponding adjustments according to the situation.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.