English
Fleascáin chultúir cillea úsáidtear go príomha le haghaidh cultúr cille beag agus meánmhéide, mar sin cén cineál cealla is féidir a shaothrú i bhfleascáin chultúir cille?
Tá go leor cineálacha cealla ann, atá roinnte i gcealla greamaitheacha agus cealla fionraí de réir a bpatrúin fáis. Ní mór go mbeadh dromchla tacaíochta ag fás na gcealla greamaitheacha ar féidir a cheangal leis, agus bíonn na cealla ag brath ar fhachtóirí ceangail atá secreted acu féin nó a chuirtear ar fáil sa mheán cultúir. D'fhonn fás agus atáirgeadh ar an dromchla seo. Níl fás na gcealla fionraí ag brath ar dhromchla na tacaíochta agus fásann sé i bhfionraí sa mheán cultúir. Go ginearálta déantar fleascáin chultúir cille d'amhábhair trédhearcacha polaistiréin. I measc na sonraíochtaí coitianta tá 25cm2, 75cm2, 175cm2, agus 225cm2. Tá dromchla bun an fhleascáin cille deartha le cruth stua chun uillinneacha marbh teagmhála a laghdú nuair a bhíonn cealla á phíobáil. Is féidir é a úsáid le haghaidh cultúr na gcealla greamaitheacha agus cultúr statach na gcealla fionraí araon. Is iad seo a leanas na cineálacha cille is infheidhme:
Cealla greamaitheacha: cealla Vero, cealla HEK 293, cealla CAR-T, MRC5, cealla CEF, macrophages alveolar muc, cealla myeloma, cealla DF-1, cealla ST, cealla PK15, Marc145 cealla, etc.
Cealla fionraí: cealla CHO, cealla feithidí, cealla BHK21 agus cealla MDCK, etc.
Ba chóir a thabhairt faoi deara má úsáidtear an fleascáin chultúir cille chun cealla greamaitheacha a shaothrú, dromchla an ní mór fleascán a mhodhnú go speisialta chun a hydrophilicity a mhéadú chun freastal ar shaintréithe fáis na gcealla greamaitheacha. Nuair a úsáidtear é i gcultúr cille fionraí, níl aon chóireáil speisialta ag teastáil.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.