English
Cell Factoryis cultúr cille ilchisealach inchaite atá oiriúnach do chultúr cille ar scála mór. Chomh maith le timpeallacht shonrach do chultúr na gcealla, is réiteach riachtanach é sú díleácha freisin.
I gcultúr in vitro na gcealla fíocháin agus scaipeadh cealla fíocháin i gcultúr na gcealla príomhúla (ullmhaítear an bloc fíocháin isteach i bhfionraí cille aonair) agus san fhochultúr, ba cheart go n-úsáidfeadh díleá agus scaipeadh cealla fáis greamaitheacha tuaslagán díleácha cille fíocháin. Is é an réiteach díleá a úsáidtear i dteicneolaíocht cultúir mhonarcha cealla ná trypsin, EDTA, etc. Is féidir le trypsin in trypsin próitéiní a thiontú ina peptides, agus is féidir le lipáis pancreatach saill a dhianscaoileadh i gliocról agus aigéid sailleacha, rud a scarann cealla. Cealla a lysed. Is é feidhm an tuaslagáin díleá den chuid is mó ná na próitéiní idircheallacha (cosúil le maitrís extracellular) a hidrealú, na fíocháin nó na cealla greamaitheacha a scaipeadh i gcealla aonair, agus fionraí cille a dhéanamh le haghaidh tuilleadh turgnaimh.
Tá ról tábhachtach ag einsímí díleácha. i gcultúr na monarchana cille, ach ba chóir a thabhairt faoi deara go bhfuil freagraí difriúla ag cealla éagsúla ar einsímí pancreatacha. Nuair a bhíonn an luach pH 8.0 agus an teocht 37 ° C, tá an éifeacht is láidre ag an réiteach einsím pancreatach. , ba cheart an tiúchan, an teocht agus an t-am a rialú go maith nuair a úsáidtear iad, ionas go seachnófar díleá iomarcach a dhéanann difear do chloí agus d'fhás cealla.
Digestive enzymes play an important role in the culture of cell factories, but it should be noted that different cells have different responses to pancreatic enzymes. When the pH value is 8.0 and the temperature is 37 °C, the pancreatic enzyme solution has the strongest effect. , the concentration, temperature and time should be well controlled when using, so as to avoid excessive digestion affecting the adherence and growth of cells.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.