English
Is cothaitheach riachtanach é meánach sa phróiseas de chultúr cille. Nuair a bhíonn cell factory á úsáid le haghaidh cultúr cille, is gnách go mbíonn oibríocht mheán-mhalartaithe i gceist leis, a fhéadfaidh an dramhaíl meitibileach a tháirgeann na cealla a bhaint le linn an phróisis fáis, agus an meán úr a athlánú chun na cealla a fhás agus a atáirgeadh níos fearr.
Chun an meán a athsholáthar sa mhonarcha cille, ullmhaigh an trealamh riachtanach ar dtús: is féidir le huisciú alcóil a líonadh le 75% alcól steiriliú leighis, maolán PBS, meán ina bhfuil serum, clúdach aistrithe leachtach, feadán aistrithe, umar dramhaíola, etc. Ansin cuir an trealamh go léir isteach sa bhinse ultra-ghlan agus cas ar an ionradaíocht UV le haghaidh steiriliú. Má tá na cealla saothraithe íogair do theocht, is féidir leat an buidéal meánach ina bhfuil séiream a chur isteach i ndabhach uisce ag 37 ° C, ionas go mbeidh teocht an mheáin ag 37 ° C, agus ansin aistriú chuig an mbinse ultra-ghlan le haghaidh UV. steiriliú, agus tá gá le ionradaíocht UV ar feadh thart ar leath uair an chloig idir cultúir na gcealla. le canna uisce alcóil le haghaidh díghalrúcháin, agus é a aistriú chuig an mbinse ultra-ghlan, d'oscail an clúdach, caipín aistrithe leachtach ceangailte agus feadán aistrithe chuig meán cultúir onnmhairithe. Cuir meán úr ina bhfuil serum leis, bí cúramach gan é a chur ó dhromchla greamaitheach na gcealla, ach ón taobh-bhalla, agus bogadh go réidh chun níocháin na gcealla a sheachaint. Tar éis an meán a chur leis, clúdaigh an clúdach, marcáil dromchla na n-earraí inchaite, agus cuir in iúl an t-am athraithe meánach, cineál cille, oibreoir agus faisnéis eile. Sula gcuirfidh tú isteach sa ghorlann é, spraeáil an dromchla le halcól arís, slacht a chur ar an tábla oibriúcháin, wipe an tábla ultra-ghlan le halcól, agus leacht dramhaíola agus truflais a ghlanadh.
Tá cealla íogair don chomhshaol. Nuair a bhíonn an meán á athrú sa mhonarcha cille, ní mór aird a thabhairt ar oibriú aseptach chun tionchar a imirt ar fhás cille de bharr ionfhabhtú baictéir éagsúla a sheachaint le linn an phróisis athraithe mheán.
Cells are sensitive to the environment. When changing the medium in the cell factory, attention must be paid to aseptic operation to avoid affecting cell growth due to infection of various bacteria during the medium change process.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.