English
Is teicneolaíocht í an teicneolaíocht chultúir cille a úsáidtear go coitianta i réimsí na bithchógaisíochta, antasubstaintí monaclónacha, agus teiripe cille. Úsáidtear Cellfactory go príomha le haghaidh cultúr cille ar scála mór. Bíonn tionchar ag go leor fachtóirí ar fhás na gceall, ar gné an-tábhachtach í an teocht.
Faoi ghnáththosca, is é 37-38 °C an teocht oiriúnach do chultúr in vitro na gcealla mamacha agus éanlaithe. Cuirfidh teocht ró-ard nó ró-íseal isteach ar fhás cealla. Tá cumas na gcealla chun teocht íseal a fhulaingt níos láidre ná cumas friotaíochta teasa. Ag teocht íseal, laghdaítear gníomhaíocht meitibileach agus rannán núicléach na gcealla. Nuair nach bhfuil an teocht níos ísle ná 0 ° C, cé go gcuireann sé isteach ar mheitibileacht cille, níl aon éifeacht díobhálach aige; nuair a chuirtear na cealla ag 25 go 35 ° C, is féidir leis na cealla maireachtáil agus fás fós, ach tá an luas moilliú; Ar ais go dtí 37 ℃ Is féidir le cealla cultúr ar aghaidh ag fás.
Níl an teocht ard go maith do chultúr na cille. Déantar cealla a shaothrú ag 39-40 ° C ar feadh 1 uair an chloig, is féidir iad a mhilleadh go pointe áirithe, ach is féidir leo a ghnóthú go fóill, ach ní féidir leo méadú teochta 2 ° C a fhulaingt ar feadh roinnt uaireanta, is é sin, má tá siad saothraithe. ag 41-42 ° C ar feadh 1 uair an chloig, tá an damáiste cille dian. Maraíodh an chuid is mó de na cealla nuair a bhí an teocht os cionn 43 °C. Is cúis le teocht ard go príomha díghníomhú einsímí, scrios lipidí, scrios rannán núicléach, táirgeadh téachta, téachtadh cealla, agus dínádúrú próitéiní. Mar sin, ní mór don mhonarcha cealla teocht oiriúnach a choinneáil agus teocht ard a sheachaint nuair a bhíonn cealla á saothrú. Áirítear le daoine eile brú osmotic, timpeallacht steiriúil, gás, agus pH.
In conclusion, cell growth in a cell factory is affected by multiple factors, and temperature is only one of them. Others include osmotic pressure, sterile environment, gas, and pH.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.