English
Nuair a bhíonn cealla éagsúla á saothrú, is cineál tomhaltáin saothraithe cille iad fleascanna cultúir cille a úsáidtear níos minice. Úsáidtear iad den chuid is mó le haghaidh cultúr na gcealla greamaitheacha, agus raon na sonraíochtaí ó 25ml go 225ml. Bainfidh meán cultúir na gceall duán hamster úsáid as an inchaite seo, agus is iad seo a leanas céimeanna sonracha a chultúir:
1. Obair ullmhúcháin do chultúr na gceall:
1) Meán MEM a ullmhú; serum bó-ainmhithe féatais ard-chaighdeán, 10%; antashubstaint dhúbailte 1%.
2) Coinníollacha cultúir: Céim gháis: aer, 95%; dé-ocsaíd charbóin, 5%. Teocht: 37 céim Celsius, bogthaise gorlainne de 70% -80%.
3) Réiteach reo: 90% serum, 10% DMSO, réidh le húsáid.
2. Próiseáil cille:
1) Aisghabháil cealla crióchaomhnaithe: Croith go tapa agus leáigh an feadán crióchoimeádaithe ina bhfuil 1 ml d'fhionraí cille i ndabhach uisce 37 ° C, cuir le feadán lártheifneoir é ina bhfuil 4-6 mL de mheán iomlán agus meascán go maith. Lártheifneoir ag 1000RPM ar feadh 3-5 nóiméad, caith amach an comhshnámh, agus cuir na cealla ar fionraí i meán iomlán. Cuireadh fionraí na cille ansin le fleascán saothraithe cille ina raibh 6-8 ml de mheán iomlán agus saothraithe ag 37°C thar oíche. Breathnaíodh fás cille agus dlús cille faoi mhicreascóp an lá dár gcionn. cealla, tá sé molta a reo bhaisc síolta cille sa chéad 3 pasáistí de chultúr le haghaidh turgnaimh ina dhiaidh sin.
Is iad na thuas na céimeanna sonracha le haghaidh saothrú cealla duáin hamster i bhfleascáin cultúr cille. Ina theannta sin, nuair a bhíonn cealla cryopreserved, bailigh na cealla díleáite isteach i bhfeadán lártheifneoir de réir an phróisis sliocht cille, agus bain úsáid as hemocytometer chun iad a chomhaireamh chun dlús cryopreservation na gcealla a chinneadh. Is é an dlús reo molta do chealla ginearálta 1 × 106 ~ 1 × 107 cealla inmharthana / ml.
3) Cryopreservation of cells: After receiving the cells, it is recommended to freeze a batch of cell seeds in the first 3 passages of culture for subsequent experiments.
The above are the specific steps for culturing hamster kidney cells in cell culture flasks. In addition, when cells are cryopreserved, collect the digested cells into a centrifuge tube according to the process of cell passage, and use a hemocytometer to count them to determine the cryopreservation density of cells. The recommended freezing density of general cells is 1×106~1×107 viable cells/ml.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.