English
Is cealla greamaitheacha an chuid is mó de na cealla saothraithe in vitro. Caithfidh dromchla tacaíochta a bheith ag fás cealla den sórt sin is féidir a cheangal leis. Is féidir le cealla fás agus iolrú ar an dromchla trí bheith ag brath ar fhachtóirí ceangail atá secreted acu féin nó a chuirtear ar fáil sa mheán cultúir. Mar sin, cad iad na ceanglais maidir le hearraí inchaite saothraithe cille de chealla greamaitheacha?
Ní mór na cealla atá ag brath ar ancaireacht a cheangal le balla an tsoithigh cultúir (buidéal) nuair a bhíonn siad saothraithe. Nuair a bheidh na cealla ceangailte, leathnaíonn siad go tapa, ansin tosaíonn siad mitosis, agus téann siad isteach go tapa sa chéim fáis logartamach. Tar éis cúpla lá, clúdóidh na cealla dromchla an tsoithigh cultúir agus foirmfidh siad monolayer dlúth cille. I measc na n-ábhar inchaite cultúir cille a úsáidtear le haghaidh cealla greamaitheacha tá monarchana cille, plátaí cultúir cille, fleascáin spinner cille, etc. Faoi láthair, is ábhair pholaiméire den chuid is mó mar PS, PC, agus PE iad na táirgí inchaite cille ar an margadh. Níl na hairíonna hydrophilic gloine ag an gcineál seo ábhar, agus ní mór an dromchla a chóireáil go speisialta sular féidir é a úsáid chun cealla greamaitheacha a shaothrú. Tá grúpa hydrophilic tugtha isteach ar an dromchla ag na hábhair inchaite cultúir cille atá cóireáilte go speisialta, rud a ligeann do chealla cloí níos fearr le dromchla na n-ábhar inchaite, rud a chabhródh le fás cille agus atáirgeadh.
Éilíonn fás cille timpeallacht shonrach, mar shampla carbóin dé-ocsaíde, pH, steiriúlacht, brú osmotic, etc., mar sin ní mór ábhair inchaite sa chultúr cille a steiriliú agus coinníollacha saor ó DNase agus RNase a bhaint amach sular féidir iad a úsáid, chun éilliú cille a sheachaint a dhéanann difear don chultúr cille dul chun cinn.@ Is é __@Surface TC cóireáil agus steiriliú an dá bhunriachtanas maidir le hábhair inchaite cultúir cille do chealla greamaitheacha, agus tá siad ina réamhriachtanais freisin chun dul chun cinn rianúil a dhéanamh ar obair chultúir cille. Dá bhrí sin, ní mór duit a bheith níos aireach nuair a roghnaíonn tú táirgí inchaite.
Surface TC treatment and sterilization are the two basic requirements for cell culture consumables for adherent cells, and they are also prerequisites for the smooth progress of cell culture work. Therefore, you need to be more cautious when choosing consumable products.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.