English
Is struchtúr aon-chiseal nó ilchiseal d'ábhair inchaite shaothrúcháin cille é cell factory. Le linn phróiseas cultúir na gceall, ní mór dúinn staid fáis na gcealla a thuiscint in am sula dtéann muid ar aghaidh go dtí an chéad chéim eile. Is féidir líon na gceall inmharthana a bhrath le staining gorm trypan. Is é seo a leanas an modh oibríochta sonrach:
1. Maidir le cealla greamaitheacha, asú an mheán, cuir an tuaslagán díleá, agus díolama sa ghorlann ar feadh tréimhse chuí. Tar éis do na cealla a bheith cruinn, cuireadh deireadh leis an díleá trí mheán toirte comhionann nó níos mó ná toirt an díleáite a chur leis. Tar éis lártheifneoraithe, déantar an fionraí cille le meán cultúir;
2. Glac tuaslagán gorm trypan 0.5ml, fionraí cille 0.3ml PBS agus 0.2ml agus measc go maith. Ansin fágadh an meascán ar feadh 1-2min. Úsáid pípéad ribeach nó barr pípéad 10ul beag chun méid beag den mheascán a tharraingt agus instealladh isteach sa haemocytometer é;
3. Déan 1000 cill ar a laghad a chomhaireamh agus líon na gceall daite a chomhaireamh;
4. Nigh an pláta comhairimh agus an duillín clúdaigh le huisce ultrapure agus 75% eatánól;
5. Ní dhéanfar gnáthchealla a dhaite, ach amháin tar éis na cealla bás, éiríonn an tréscaoilteacht membrane cille níos mó, téann trypan gorm isteach sna cealla, agus bíonn na cealla gorm le feiceáil. Níor cheart an meascán a chur ar feadh ró-fhada, nó beidh na gnáthchealla beo ag gabháil leis an dath freisin, rud a chuirfidh isteach ar chruinneas an chomhairimh;
6. Inmharthanacht cille (%) = (líon iomlán na gceall - líon na gceall daite)/líon iomlán na gceall*100;
7. Cosúil leis an modh comhaireamh cille, tá earráid mhór ag staining gorm trypan. Ní féidir leis ach an leibhéal apoptosis luath a bhrath, agus ní féidir leis an difríocht idir an grúpa rialaithe agus an grúpa turgnamhach a thaispeáint. Is oibríocht thábhachtach é máistreacht a dhéanamh ar líon na gcealla beo agus inmharthanacht cille i dturgnaimh chultúir cille agus bealach éifeachtach chun dul chun cinn an turgnaimh a chinntiú.
In the process of culturing cells in a cell factory, there are always some cells that die due to various reasons. Mastering the number of living cells and cell viability is a key operation in cell culture experiments and an effective way to ensure the progress of the experiment.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.