English
Fleascáin chultúir cilleIs cineál earraí inchaite saothraithe cille iad a úsáidtear go coitianta i gcultúr cille greamaitheacha. Tá cealla greamaitheacha difriúil ó chealla fionraí agus ní mór iad a cheangal le dromchla tacaíochta chun fás. Bíonn tionchar díreach ag éifeacht cloí ar fhás na gcealla, agus is féidir éifeacht cloí na gcealla a fheabhsú trí na modhanna seo a leanas:
1. Díleá cuí cealla: Is féidir le cealla ailse HepG-2, A549, Hela, SGC-7901 a chóireáil ar feadh tréimhse níos faide ama. Go ginearálta, is é 5-6 nóiméad an t-am cóireála. Tá C2C12, COS-7, NIH-3T3 níos éasca le titim amach, agus is leor 2 nóiméad. , ní chloíonn P19Cl6 agus 293 cealla go docht, ionas gur féidir iad a athsholáthar le meán úr go díreach tar éis sruthlú i PBS chun iad a scaipeadh.
2. Bain úsáid as buidéil phlaisteacha cultúir cille oiread agus is féidir. Tar éis cóireáil a dhéanamh ar bhuidéil den sórt sin le TC, is féidir feidhmíocht greamaitheachta na buidéil a fheabhsú go mór, agus is fearr na cealla a cheangal leo.
3. Athbheochan cealla nua agus úsáid séiream 20% chun cabhrú le cealla téarnamh sa chéad seachtain.
4. Úsáid maitrís eischeallach cuí nó imoibrithe greamaitheachta chun cabhrú le cealla cloí níos fearr leis an mballa.
5. Déan iarracht gan na cealla a chroitheadh taobh istigh de 24 uair an chloig tar éis an pasáiste, ionas nach gcuirfear isteach ar éifeacht greamaitheachta cille.
6. Fásann cealla epithelial sa chorp ar an membrane íoslach déanta as collagen, agus mar sin tá saothrú ar an tsubstráit le collagen tairbheach don fhás. Maidir le cultúr cille eipideirm daonna nó luch, is féidir cealla 3T3 atá ionradaithe le γ-ghathanna a úsáid mar chiseal friothálacha, agus laghdaítear an t-ábhar pH, Ca2+ agus an teocht, rud atá tairbheach d'fhás cealla epithelial.
Tá roinnt bealaí ann. feabhas a chur ar éifeacht greamaitheachta na gcealla sa cell culture flask. Ina theannta sin, a bheith cinnte a scaipeadh go cothrom le linn subculture a sheachaint fás clumping cille
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.