English
2. Ábhair agus Modhanna
2.1. Coinníodh cultúir cille Huh7.5 Cells
Huh7.5 i cultúr cille T fleascáin i meán ina bhfuil séiream (SCM): Meán Iolair Athraithe Dulbecco (DMEM) (Gibco, Paisley, RA) ina raibh 4 mM GlutaMAX agus forlíonta leis Séiream buaibheach féatais 10% (Sigma, St. Louis, MO, SAM), 100 U/mL peinicillin agus 100 µg/mL streiptimícin (Sigma). I bhfleascáin T175 (Nunc, Roskilde, an Danmhairg) agus i bhfleascáin shaothrúcháin thríchiseal (Nunc), rinneadh cealla Huh7.5 a thrasnú gach 2-3 lá. Coinníodh cultúir na gceall go léir ag 37 °C agus 5% CO2.
2.2. Stoic Víreas HCV
Baineadh úsáid as dhá stoc seicheamh-dheimhnithe den ghéinitíopa 5a athchuingreach SA13/JFH1core-NS5B [28], a fhástar i gcultúr cille monolayer Huh7.5 i bhfleascáin T, sa staidéar seo. Baineadh úsáid as stoc víreas tríú pasáiste le titer ionfhabhtaithe de 5.8 log10 FFU/mL, arna fhás faoi choinníollacha ina bhfuil séiream (SC) [39], chun cultúir CelCradle ™ a ionaclú, cultúir fhleascáin a chroitheadh, agus le haghaidh turgnaimh tréithrithe víreas. Baineadh úsáid as stoc víreas ceathrú pasáiste le titer ionfhabhtaithe de 5.1 log10 FFU/mL [32], arna fhás faoi choinníollacha SF [39], le haghaidh turgnaimh tréithrithe víreas.
Foinse ó: https://www.mdpi .com/2076-393X/10/2/249/htm
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.