English
Is fadhb an-choitianta é éilliú mycoplasma agus cealla á saothrú in monarchana cille. Murab ionann agus éilliúcháin eile, go ginearálta ní éiríonn cealla éillithe mycoplasma scamallach, agus mar sin tá sé deacair breithiúnas a dhéanamh leis an tsúil nocht. Tá ceithre bhealach ann lena fháil amach an bhfuil cealla truaillithe le mycoplasma:
1. staining fluaraiseacht DNA
Tá an modh staining fhluaraiseachta DNA bunaithe ar an bprionsabal gur féidir leis an dath fluaraiseachta Hoechst33258 ceangal a dhéanamh leis an réigiún bonn-saibhir AT i DNA an mhiocoplasma. Is éard atá sna poncanna fluaraiseacha ná mycoplasma DNA saibhir i mbunréigiúin AT.
2. PCR technology
Deartar na primers sonracha de réir na seichimh chaomhnaithe sa genome Mycoplasma, aimplítear aigéad núicléach an tsampla atá le tástáil, agus déantar an diagnóis trí anailís a dhéanamh ar mhéid an táirge aimplithe. Úsáidtear teicneolaíocht braite PCR chun éilliú mycoplasma a bhrath, le timthriall gearr, íogaireacht ard, sainiúlacht mhaith, oibriú simplí, agus féadann sé líon mór samplaí a bhrath ag aon am amháin.
3. Baintear úsáid as measúnacht imdhíon-ionsorbent einsím-nasctha (ELISA)
ELISA chun éilliú mycoplasma a bhrath, le sainiúlacht agus le híogaireacht mhaith, agus féadann sé líon mór samplaí a bhrath ag aon am amháin. Tá tréithe braite cáilíochtúil simplí, thapa agus chainníochtúil aige.
4. Leictreonmhicreascóp
Go ginearálta, saothraítear cealla i monarchana ceall ar feadh 48 go 72 uair an chloig. Sula mbíonn na cealla beagnach comhlíonta, déantar na cealla a dhíleá le trypsin chun fionraí cille, seasta, leabaithe, agus slisnithe roimh bhreathnóireacht. Faoi láthair, tá níos mó ná 20 cineál mycoplasma ar eolas go n-éilíonn cealla, a bhfuil níos mó ná 95% díobh ina mycoplasma béil, mar sin ba chóir d'oibreoirí aird a thabhairt ar oibriú aiseipteach agus cealla á saothrú.
The above are four commonly used detection methods when detecting mycoplasma contamination in cell factories. At present, there are more than 20 kinds of mycoplasma known to contaminate cells, of which more than 95% are oral mycoplasma, so operators should pay attention to aseptic operation when culturing cells.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.