English
Cell factoryis modh cultúir a úsáidtear go coitianta é teicneolaíocht cultúir do go leor cultúir ar scála mór. Agus cealla á shaothrú, beidh tionchar ag go leor fachtóirí air, rud a chuireann bac ar ghnáthfhás agus atáirgeadh cealla. Tá radaíocht agus tonnta ultrasonaic ar cheann acu.
Solas infheicthe: Is é tonnfhad an tsolais infheicthe ná 390~780nm. Is féidir le solas daite éagsúla a bheith ina chúis le díghiniúint cille, síneadh a chur leis an idirphase de rannán núicléach, agus laghdaítear go mór cumas na gceall chun gabháil leis an mballa. Dá bhrí sin, nuair a úsáidtear an mhonarcha cealla le haghaidh cultúr cealla in vitro, ba cheart solas díreach a sheachaint, agus ba cheart an cultúr a dhéanamh sa dorchadas nó a stóráil ar feadh tréimhse ghearr ama a oiread agus is féidir.
ghathanna ultraivialait: Cealla nach bhfuil an-fhulangach do ghhathanna UV laga athrú mórán, ach déantar damáiste do chealla íogair. Nuair a bhíonn na gathanna ultraivialait láidir, léiríonn na cealla scoite: nach féidir miotóis iomlán a dhéanamh; méadaítear syneresis le linn mitosis; laghdaítear blebbing cytoplasmic le linn miotóis. Cruthaíonn blisters ar an dromchla ionradaithe, agus ina dhiaidh sin tá leathnú cille agus damáiste níos mó. rannáin núicléacha neamhghnácha a chur faoi deara, rud a d'fhéadfadh bás cille a chur faoi deara.
Ultrafhuaim: Faoin gcreathadh ultrasonaic, réabfaidh na cealla go luath, agus beidh an cíteaplasma ag sreabhadh i neamhord ar dtús, agus athróidh struchtúr colóideach an protoplasm go suntasach freisin. Má stoptar an tonnchrith ultrasonaic, is féidir é a chur ar ais. Is é an chúis le bás cille mar gheall ar cavitation. Nuair a bhíonn an tonn ultrasonaic ag 2.5W/cm2, déantar damáiste do na cealla agus tá na crómasóim neamhbhuan. Is iad na crómasóim núicléacha na chéad cinn atá saobhadh. Ina theannta sin, beidh tionchar ag fachtóirí cosúil le teocht, brú osmotic, agus luach pH ar fhás agus atáirgeadh cealla freisin. Is féidir gnáthfhás cille a chinntiú trí na fachtóirí seo a rialú.
Ultrasound: Under the ultrasonic vibration, the cells will soon rupture, and the cytoplasm will flow in disorder at first, and the colloidal structure of the protoplasm will also change significantly. If the ultrasonic vibration is stopped, it can be restored. The cause of cell death is due to cavitation. When the ultrasonic wave is at 2.5W/cm2, the cells are damaged and the chromosomes are aberrant. The nuclear chromosomes are the first to be distorted.
Radiation and ultrasonic waves are important factors when using cell factories for cell culture. In addition, factors such as temperature, osmotic pressure, and pH value will also affect the growth and reproduction of cells. Controlling these factors can ensure normal cell growth.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.