Go ginearálta, ba cheart cealla le meitibileacht láidir agus fás tapa (cosúil le cealla meall) a ionaclú ag tiúchan níos ísle; cé go bhfásann gnáthchealla fíocháin go mall agus nach bhfuil a meitibileacht bríomhar go leor, féadfaidh an tiúchan ionaclaithe a bheith níos airde; Le húsáid éigeandála, is féidir an ionaclú a bheith níos ísle; uaireanta chun breathnú ar mhoirfeolaíocht na gcealla a éascú, is féidir an tiúchan ionaclaithe a laghdú go cuí freisin. Is féidir gnáthchealla a shíolú de réir líon na gcealla in aghaidh an aonaid achair den mhonarcha cealla, arna iolrú faoi limistéar cultúir an mhonarcha cealla a úsáidtear, agus ansin roinnte ar an gcóimheas síolaithe de chealla saothraithe. Ní chabhródh méid ionaclaithe cealla ró-ard nó ró-íseal le hatáirgeadh cille. Is é an méid ionaclaithe cille cuí an bonn chun gnáthfhás na gcealla a chinntiú. Ach amháin trí rialú a dhéanamh ar an dlús cuí is féidir an turgnamh cultúir cille a dhéanamh go réidh.tá ról tábhachtach ag teicneolaíocht cultúir i réimsí ullmhúcháin antashubstaint mhonaclónacha agus vacsaíní. Nuair a bheidh cealla á saothrú, beidh tionchar ag fachtóirí éagsúla air, ina measc beidh tionchar ag an dlús ionaclaithe cille ar phróiseas cultúir na gcealla. tá tionchar díreach aige ar fhás na gcealla. Is féidir le dlús ionaclaithe cuí iomadú cille níos fearr a chur chun cinn. Ní chabhródh dlús ionaclaithe ró-ard nó ró-íseal le fás agus iomadú cille. Ba cheart conas an tiúchan ionaclaithe cuí a roghnú a chinneadh de réir meitibileacht cille, luas fáis agus atáirgeadh agus riachtanais na hoibre.
When using cell factories for large-scale cell culture, the number of cell inoculations has a direct impact on the growth of cells. An appropriate inoculation density can promote better cell proliferation. Too high or too low inoculation density is not conducive to cell growth and proliferation. How to choose the appropriate inoculation concentration should be determined according to the cell metabolism, growth and reproduction speed and the needs of the work.
Generally speaking, cells with strong metabolism and fast growth (such as tumor cells) should be inoculated at a lower concentration; while normal tissue cells grow slowly and their metabolism is not vigorous enough, the inoculation concentration may be higher; For emergency use, the inoculation can be lower; sometimes in order to facilitate the observation of cell morphology, the inoculation concentration can also be appropriately reduced. Ordinary cells can be seeded according to the number of cells per unit area of the cell factory, multiplied by the cell factory culture area used, and then divided by the seeding ratio of cultured cells. Too high or too low inoculation amount of cells is not conducive to cell reproduction. Appropriate cell inoculation amount is the premise to ensure the normal growth of cells.
The cell seeding density is an important factor affecting the cultured cells of the cell factory. Only by controlling the appropriate density can the cell culture experiment be carried out smoothly.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.