English
Teagmhas marú ceall amháin is ea citotocsaineacht de bharr cealla nó ceimiceáin nach bhfuil ag brath ar apoptosis nó neacróis mar mheicníocht bás cille. Tríd an tástáil seo, is féidir a mheas an bhfuil substaintí in amhábhair an 1. Modhanna MTT agus XTT: ag baint úsáide as gníomhaíocht na n-einsímí taobh istigh den mitochondria, is féidir salainn tetrazolium ar leith a thiontú, agus ansin a bhrath ag léitheoir micreaphláta; nach bhfuil a chuidíonn le fás cille. /nó próiseas fiseolaíoch na gceall, mar chillscannán nó struchtúr cítea-chnámharlaigh, próiseas meitibileachta na gceall, sintéis, díghrádú nó scaoileadh comhpháirteanna cille nó táirgí, rialáil ian agus roinnt ceall agus próisis eile, as a dtagann neamhoird ar mharthanas, iomadú agus/nó ceall. feidhm, a eascraíonn i frithghníomhartha díobhálacha. Go ginearálta, is féidir na modhanna seo a leanas a thástáil an bhfuil monarcha ceall cítotocsaineach:
2. Modh LDH: aimsítear cíteatocsaineacht trí ghníomhaíocht einsíme LDH a bhrath i gcultúr na cille;
3. Modhanna einsímeacha eile: amhail gníomhaíochtaí fosfatase alcaileach agus fosfatase aigéid a bhrath san fhorshuíomh;
4. Kit Anailís Iomadú Cille: Tá meitibileacht bríomhar ag gnáthchealla, agus is féidir le succinate dehydrogenase ina mitochondria salainn tetrazolium (cosúil le MTT, XTT, WST-1, etc.) a laghdú go substaintí criostalach corcra, a thaisceadh timpeall na gcealla, agus ansin léigh an OD luach ag léitheoir micreaphláta chun staid iomadú cille a bhrath.
5. Inmharthanacht cille a bhrath trí mhodh luminescence fluorescein: Tá adenylate kinase (AK) ann i gcíteaplasma na gceall eocaryotic agus procaryotic go léir, agus is féidir le AK ADP a ghníomhachtú chun ATP a ghiniúint. Nuair a dhéantar damáiste do chealla, déantar damáiste don chillscannán agus scaoiltear AK isteach sa chultúir supernatant. Úsáideann an trealamh luciferase agus luciferin chun solas a astú faoi ghníomh ATP, agus is féidir é a bhrath go cainníochtúil le hionstraim chemiluminescence.
Is féidir tástáil an bhfuil cíteatocsaineacht ag monarcha ceall a dhéanamh ar bhealaí éagsúla, agus tá an tástáil seo ar cheann de na cinn freisin. na míreanna tástála gnáthaimh chun cáilíocht na n-ábhar inchaite saothraithe cille a chinntiú.
5. Detection of cell viability by fluorescein luminescence method: Adenylate kinase (AK) exists in the cytoplasm of all eukaryotic and prokaryotic cells, and AK can activate ADP to generate ATP. When cells are damaged, the cell membrane is damaged and AK is released into the culture supernatant. The kit uses luciferase and luciferin to emit light under the action of ATP, and can be quantitatively detected by a chemiluminescence instrument.
Testing whether a cell factory has cytotoxicity can be carried out in several ways, and this test is also one of the routine testing items to ensure the quality of cell culture consumables.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.