English
Is struchtúr ilchiseal d'ábhair inchaite cultúir cille é an Ní hamháin go bhfuil dé-ocsaíd charbóin ina mheitibilít de cealla, ach freisin ina chomhpháirt riachtanach d'fhás cille, agus tá baint aige le pH an chultúir a chothabháil. I gcás tiúchan dé-ocsaíd charbóin íseal i dtimpeallacht dúnta, tá cealla éasca le fás; go ginearálta ní féidir leis a bheith níos ísle ná 1%, ar shlí eile déanfar damáiste do na cealla. Má chuirtear an cultúr oscailte i dtimpeallacht gháis ina bhfuil 5% dé-ocsaíd charbóin, tá sé inmholta., a úsáidtear go príomha le haghaidh cultúr cille in vitro ar scála mór. Nuair a bhíonn cealla á saothrú, tá gá le timpeallacht shonrach, agus tá an timpeallacht gáis agus luach pH ar cheann de na coinníollacha is tábhachtaí. ocsaigine. Glacann ocsaigin páirt i dtimthriall aigéad tricarboxylic na cille chun fuinneamh a ghiniúint le haghaidh fás cille, iomadú agus sintéis na gcomhpháirteanna riachtanacha éagsúla. Is féidir le roinnt cealla fuinneamh a fháil trí glicealú faoi choinníollacha hipocsaíocha, ach ní féidir leis an gcuid is mó de na cealla maireachtáil faoi hypoxia. De ghnáth coinnítear teannas ocsaigine ag beagán faoi bhun na gcoinníollacha atmaisféaracha, agus má sháraíonn brú páirteach ocsaigine an méid ocsaigine san atmaisféar, d'fhéadfadh go mbeadh roinnt cealla díobhálach. Nuair a úsáidtear cultúr oscailte (cultúr caipín scaoilte buidéal mias nó cultúir nó cultúr pláta cultúir), cuirtear na cealla go ginearálta i dtimpeallacht gháis mheasctha de 95% aeir agus 5% dé-ocsaíd charbóin.
Tá an chuid is mó de na cealla oiriúnach le haghaidh fáis ag pH 7.2 ~ 7.4, níos ísle ná pH 6.8 nó níos airde ná pH 7.6 díobhálach do chealla, agus fiú degenerate nó bás. Tá ceanglais dhifriúla ag cealla éagsúla maidir le luach pH. Go ginearálta, tá cealla cultúir bunscoile níos lú fulangach le luach pH alcaileach ná caoinfhulaingt aigéad, agus tá timpeallacht aigéadach níos tairbhí d'fhás cille ná timpeallacht alcaileach. D'fhonn luach pH leanúnach na timpeallachta cultúir a choinneáil, is minic a úsáidtear modh maolán cosúil le fosfáit a chur leis an meán.
Le forbairt go mear ar réimse na heolaíochta beatha, tá monarchana cille á gcur i bhfeidhm níos mó. páirceanna. Ach is cuma cén réimse ina n-úsáidtear é, tá an timpeallacht gáis agus luach pH le linn chultúr na gceall fós ar cheann de na coinníollacha tábhachtacha maidir le fás cille.
Most cells are suitable for growth at pH 7.2~7.4, lower than pH 6.8 or higher than pH 7.6 are harmful to cells, and even degenerate or die. Different cells have different requirements for pH value. Generally, primary culture cells are less tolerant to alkaline pH value than acid tolerance, and an acidic environment is more beneficial to cell growth than an alkaline environment. In order to maintain a constant pH value of the culture environment, a method of adding a buffer such as phosphate to the medium is often used.
With the rapid development of the field of life science, cell factories are being applied to more fields. But no matter which field it is used in, the gas environment and pH value during cell culture are still one of the important conditions for cell growth.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.