English
Is gnáthchultúr cille inchaite é an fleascáin chultúir, ar féidir a úsáid le haghaidh cultúr na gcealla greamaitheacha agus cultúr na gceall fionraí. I measc a n-iliomad feidhmchlár, is catagóir sách mór iad cealla meall. Ar an gcéad dul síos, tá cealla ailse sách éasca le cultúr. Tá go leor línte cille ailse sna línte cille atá bunaithe faoi láthair. Ina theannta sin, is iad tumaí an galar is bagairt do dhaoine. Is modh thar a bheith tábhachtach é cultúr ceall meall chun staidéar a dhéanamh ar mheicníocht na hailse, ar bhrath drugaí frith-ailse, agus ar bhitheolaíocht mhóilíneach ailse. Beidh ról do-mheasta aige maidir le hailse a shoiléiriú agus a réiteach. Is é an rud is tábhachtaí dá rath ná roghnú na n-ábhar, deireadh a chur le fibroblasts, agus roghnú na meán cultúir agus foshraitheanna cultúir chuí. I dtéarmaí modhanna cultúir ar leith, níl aon difríocht phrionsabail idir cultúr cille meall agus gnáthchultúr cille fíocháin. Is féidir le cultúr bunscoile úsáid a bhaint as bloc fíocháin agus modhanna cultúir díleá. cuirfidh sé isteach ar fhás cealla meall nó fiú faoi chois. Is féidir deireadh a chur le fibroblasts trí scrapáil meicniúil, gabháil arís agus arís eile, díleá agus eisiamh, agus díleá collagen trypsin. Braitheann an rogha sonrach ar an gcineál cille.
Tumor cells occupy a central position in tissue culture. First of all, cancer cells are relatively easy to culture. There are many cancer cell lines in the currently established cell lines. In addition, tumors are the most threatening disease to humans. Tumor cell culture is an extremely important method for studying the mechanism of cancer, anticancer drug detection, and cancer molecular biology. It will play an inestimable role in elucidating and solving cancer.
Tumor cell culture through cell culture flasks is an important way for scientific research institutions and pharmaceutical companies to conduct drug research and disease treatment. The key to its success lies in the selection of materials, the elimination of fibroblasts, and the selection of appropriate culture media and culture substrates. In terms of specific culture methods, there is no principle difference between tumor cell culture and normal tissue cell culture. Primary culture can use tissue block and digestion culture methods.
It should be noted that when using cell culture flasks for tumor cell culture, fibroblasts often grow mixed with tumor cells at the same time, making it difficult to purify tumor cells, and it will affect or even suppress the growth of tumor cells. Fibroblasts can be eliminated by mechanical scraping, repeated attachment, digestion and exclusion, and collagen trypsin digestion. The specific choice depends on the type of cell.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.