English
Fleascáin chultúir cilleis cineál inchaite cultúir cille iad a úsáidtear go forleathan ar an margadh, agus atá oiriúnach go príomha le haghaidh cultúr cille greamaitheacha. Ina measc, úsáidtear an inchaite seo freisin le linn fibroblasts luch a aistriú.
Luch Is cealla speisialta iad fibroblasts a úsáidtear chun críocha neamhleighis cosúil le taighde eolaíoch nó feidhmeanna tionsclaíochta. Tá feidhmeanna bríomhar ag fibroblasts agus cíteaplasma basophilic lag. Tá gníomhaíochtaí soiléire sintéise próitéine agus secretion acu. Faoi choinníollacha áirithe, is féidir leo claochlú frithpháirteach a bhaint amach le cealla snáithíní. Tar éis na fibroblasts a fháil, is gá iad a chobhsú sa ghorlann ar feadh thart ar 4 uair an chloig agus ansin iad a chothú nó a thrasnú de réir dlús na cille, ag athrú an mheáin. Leanann an oibríocht pasáiste na céimeanna seo a leanas:
1. Asúigh an meán sa fhleascán cultúir cille bunaidh, sruthlaigh na cealla faoi dhó le maolán PBS, agus cuir 1 ~ 2 ml de 0.25% EDTA le haghaidh díleá trypsin (tabhair aird ar an am díleá, arna rialú de ghnáth laistigh de 1 ~ 2 nóiméad).
2. Breathnaigh ar an díleá faoin micreascóp. Nuair a chrapadh agus a chloíonn imeall na cille leis an mballa (ní mholtar díleá go dtí go snámhann na cealla), bain an trypsin, cuir 6 ~ 8ml de mheán iomlán leis, buille an ciseal cille go réidh, agus déan iarracht an ciseal cille a shéideadh agus a shéideadh. .
3. Tóg amach cuid den fhionraí cille agus aistrigh chuig fleascán nua saothraithe cille é, cuir meán iomlán cuí leis, agus cothaigh i gorlann.
4. Tabhair aird ar athrú luach pH an mheáin, athraigh an meán go rialta, agus déan an subculture nó an stóráil reoite arís nuair a shroicheann an dlús cille 70-80%. cealla a stóráil agus timpeallacht fáis leantach a sholáthar do chealla. Ba chóir a thabhairt faoi deara má tá an balla cille briste nó ag sceitheadh tar éis na cealla a fháil, ciallaíonn sé go bhfuil na cealla éillithe, agus ba chóir teagmháil a dhéanamh leis an soláthraí in am.
fleascáin chultúir ceallacell culture flasks in cell passaging is to store cells and provide an adherent growth environment for cells. It should be noted that if the cell wall is broken or leaking after receiving the cells, it means that the cells have been contaminated, and the supplier should be contacted in time.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.