English
1. Caith amach an bunmheán cultúir sa fhleascán saothraithe cille atá lán de chealla.tá muineál díreach, torticollis, muineál uillinn, triantán, dronuilleog, etc. de réir cruthanna éagsúla. Áirítear ar na sonraíochtaí 25ml, 75ml, 175ml, agus 250ml. Úsáidtear méideanna níos lú den chuid is mó le haghaidh pasáiste cille, caomhnú cealla, agus soláthar cealla le haghaidh turgnaimh, srl. sáithithe. D'fhonn a chumasú do na cealla leanúint ar aghaidh ag fás agus méadú ar líon na gcealla, ní mór iad a fhoshaothrú (athshaothrú). Is bealach é fochultúr freisin chun speicis cille a chaomhnú. Ag an am céanna, is próiseas riachtanach é le haghaidh turgnaimh éagsúla ag baint úsáide as cealla saothraithe. Is féidir cealla ar fionraí a scaradh go díreach, agus ní mór cealla greamaitheacha a dhíleá sular féidir iad a scaradh. Seo a leanas na céimeanna sonracha:
2. Cuir 0.5-1ml de thuaslagán pancreatin 0.25% leis, ionas go mbeidh na cealla ag bun an bhuidéil tumtha sa tuaslagán.
3. Cuir stopallán rubair ar an mbuidéal agus cuir faoi mhicreascóp inbhéartaithe é chun na cealla a bhreathnú. Le himeacht ama, de réir a chéile bhí na cealla greamaitheacha bunaidh cruinn, agus caitheadh an pancreatin nuair nach raibh na cealla ar snámh fós, agus cuireadh 10ml de mheán cultúir leis chun an díleá a fhoirceannadh. Is féidir breathnú ar dhíleá a dhéanamh freisin leis an tsúil naked. Nuair a fheiceann bun an bhuidéil bán agus pinholes fíneáil le feiceáil, is féidir an díleá a fhoirceannadh. Go ginearálta, is é an t-am díleá ag teocht an tseomra thart ar 1-3 nóiméad.
4. Bain úsáid as pípéad chun na cealla greamaitheacha a shéideadh isteach i bhfionraí, roinn iad ina dhá nó trí bhuidéil, plug an tuaslagán cultúir le stopallán rubair, agus cuir ag 37 °C é chun leanúint ar aghaidh leis an saothrú. Breathnaigh ar an bhfás greamaitheach an lá dár gcionn. Is é seo a leanas modh ullmhúcháin an tuaslagáin díleá: Meáigh 0.25g trypsin (gníomhaíocht 1:250), cuir 100ml Ca2+, Mg2+-saor ó thuaslagán Hank chun tuaslagadh, scag Steiriliú trí scagachán, stóráil ag 4°C, agus fill ar an teocht ag 37 °C roimh úsáid. Is féidir EDTA a chur leis an tuaslagán pancreatin freisin chun an tiúchan deiridh a bhaint amach 0.02%.
cell.
4. Use a pipette to blow the adherent cells into a suspension, divide them into two to three bottles, plug the culture solution with a rubber stopper, and place it at 37°C to continue culturing. Observe the adherent growth the next day.
The above is the application of cell culture flasks in cell passaging. The preparation method of digestion solution is as follows: Weigh 0.25g trypsin (activity 1:250), add 100ml Ca2+, Mg2+-free Hank’s solution to dissolve, filter Sterilize by filtration, store at 4°C, and return to temperature at 37°C before use. EDTA can also be added to the pancreatin solution to make the final concentration reach 0.02%.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.