English
2. Deunyddiau a Dulliau
2.1. Cynnal a chadw diwylliannau celloedd Huh7.5
Huh7.5 yn diwylliant cell fflasgiau T mewn cyfrwng sy'n cynnwys serwm (SCM): Eryr Addasedig Canolig (DMEM) Dulbecco (Gibco, Paisley, UK) sy'n cynnwys 4 mM GlutaMAX a'i ategu â 10% serwm buchol ffetws (Sigma, St. Louis, MO, UDA), 100 U/mL penisilin a 100 µg/mL streptomycin (Sigma). Mewn fflasgiau T175 (Nunc, Roskilde, Denmarc) a fflasgiau meithrin haen driphlyg (Nunc), roedd celloedd Huh7.5 yn cael eu pasio bob 2-3 diwrnod. Roedd pob diwylliannau cell yn cael eu cynnal ar 37 ° C a 5% CO2.
2.2. Stociau Feirws HCV
Defnyddiwyd dwy stoc o'r genoteip 5a ailgyfunol SA13/JFH1core-NS5B [28] a gadarnhawyd gan ddilyniant [28], a dyfwyd mewn diwylliannau celloedd monolayer Huh7.5 mewn fflasgiau T, yn yr astudiaeth hon. Defnyddiwyd stoc firws trydydd darn gyda thiter heintio o 5.8 log10 FFU/mL, a dyfwyd o dan amodau sy'n cynnwys serwm (SC) [39], i frechu diwylliannau CelCradle™, ysgwyd diwylliannau fflasg, ac ar gyfer arbrofion nodweddu firws. Defnyddiwyd stoc firws pedwerydd darn gyda titer heintio o 5.1 log10 FFU/mL [32], a dyfwyd o dan amodau SF [39], ar gyfer arbrofion nodweddu firws.
Ffynhonnell o: https://www.mdpi .com/2076-393X/10/2/249/htm
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.