English
Waarskynlik, wanneer navorsers selle kweek, waarvoor hulle die bangste is, is dat die selle wat so hard gekweek is, besoedel is. Vandag wil FuDau die simptome van mikoplasma-besmetting in die 10-Lae-Onbehandeld -Cell-Factory as ons dit gebruik in detail met jou deel. um en kan onafhanklik lewe. Sy grootte is tussen virusse en bakterieë. . Aangesien sy minimum deursnee ongeveer 0.2um is, is dit vergelykbaar met die porieë van hoofstroomfiltermembrane in die mark, so dit het 'n waarskynlikheid om deur die filter te gaan, en dit voel moeilik om daarteen te waak.
As die selfabriek deur mikoplasma besoedel word wanneer selle gekweek word, kan daar gevind word dat die morfologie daarvan veranderlik is wanneer dit onder 'n omgekeerde mikroskoop waargeneem word, en die meeste van hulle word op die seloppervlak en tussen selle geadsorbeer of verstrooi. . Die deursnee is soortgelyk aan sellulêre mikrovilli, maar die elektrondigtheid van mikrovilli is minder as dié van mycoplasma, en daar is geen korrelgroep of sentrale bondel in die middel nie.
Bowendien berus die metabolisme van mycoplasma-besoedeling hoofsaaklik op sterole , en sommige spesies benodig arginien, O2 of glukose. Elke mikoplasma het sy eie kenmerke, en die meeste mikoplasmas is geskik vir oorlewing onder alkaliese toestande (PH7.6-8.0), en het swak verdraagsaamheid vir suur. Sensitief vir hitte, onsensitief vir algemene antibiotika.
40-Lae-Untreated-Cell-Factory
Nadat mycoplasma selle in selkultuurverbruiksgoedere soos selfabrieke kontamineer, mag die kweekmedium in selfabrieke nie troebel voorkom nie. En in die meeste gevalle is die selveranderinge relatief gering en onbeduidend. Selfs al is daar 'n effense verandering in die selle in die selfabriek, sal dit verlig word deur deurgang en mediumvervanging, so dit is maklik om geïgnoreer te word. In ernstige gevalle kan die selle egter stadig prolifereer en selfs van die kweekvat afval.
Dit is wat FuDau in detail met jou gedeel het as ons die simptome van mikoplasma-besmetting in die selfabriek gebruik.
After mycoplasma contaminates cells in cell culture consumables such as cell factories, the culture medium in cell factories may not appear turbid. And in most cases, the cell changes are relatively slight and insignificant. Even if there is a slight change in the cells in the cell factory, it will be alleviated by passage and medium replacement, so it is easy to be ignored. However, in severe cases, the cells may proliferate slowly and even fall off from the culture vessel.
This is what FuDau shared with you in detail if we are using the symptoms of mycoplasma contamination in the cell factory.
The FAI climbed 5.9 percent year-on-year in the first 11 months of 2018, quickening from the 5.7-percent growth in Jan-Oct, the National Bureau of Statistics (NBS) said Friday in an online statement.
The key indicator of investment, dubbed a major growth driver, hit the bottom in August and has since started to rebound steadily.
In the face of emerging economic challenges home and abroad, China has stepped up efforts to stabilize investment, in particular rolling out measures to motivate private investors and channel funds into infrastructure.
Friday's data showed private investment, accounting for more than 60 percent of the total FAI, expanded by a brisk 8.7 percent.
NBS spokesperson Mao Shengyong said funds into weak economic links registered rapid increases as investment in environmental protection and agriculture jumped 42 percent and 12.5 percent respectively, much faster than the average.
In breakdown, investment in high-tech and equipment manufacturing remained vigorous with 16.1-percent and 11.6-percent increases respectively in the first 11 months. Infrastructure investment gained 3.7 percent, staying flat. Investment in property development rose 9.7 percent, also unchanged.